Fig. 6. Silencing of the cytoskeletal intermediate filament protein vimentin. Cells were transfected with vimentin duplex 2 (bp 1145-1167) (A-C) or with GL2 luciferase duplex (D). (A,B,D) Staining with vimentin V9 antibody; (C) Hoechst staining. Note the strong decrease of vimentin staining in cells transfected with vimentin duplex 2 (A) when compared with the control (D). Only at longer exposure times (B) can short filamentous structures (vimentin squiggles) (arrow) be seen. (E) Western blot using vimentin antibody (top panel), and ß-actin antibody as control (bottom panel). Magnification 460x (A-D).
