Fig. 7. Silencing of the kinesin-related motor protein Eg5 and of the microtubular motor CENP-E. HeLa cells were transfected with Eg5 siRNA (A,D,G-I) or with luciferase control siRNA (B,C,E,F). Cells are stained for 
-tubulin (A-C) with a corresponding Hoechst stain (D-F). Cells transfected with Eg5 siRNA are arrested in mitosis and show monoastral microtubular arrays (A,D). By contrast, control cells show normal bipolar spindles in mitosis (B,E) and microtubule networks in interphase (C,F). A higher magnification of cells transfected with the Eg5 siRNA clearly shows the monopolar spindle (G), which does not overlap with the chromatin staining (H); for overlay see I. HeLa cells were transfected with CENP-E siRNA (J-M). Cells were stained with -tubulin antibody (J,K) and Hoechst (L,M). Note the production of multipolar arrays. The higher magnification of a metaphase cell (K,M) indicates that the loss of CENP-E affects chromosome congression to the spindle equator (arrows). Magnification 480x (A-F,J-M); 750x (G-I).
