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Fig. 2. Blot overlay assays for emerin binding to BAF or lamin A. Bacterial lysates containing wildtype (W) or mutant emerin residues 1-222 were separated on gels, blotted and probed with: (A) anti-emerin antibodies, (B) 35S-labeled BAF, or (C) 35S-labeled lamin A. Mutants are numbered according to Fig. 1.





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