Fig. 3. Solution binding as assayed by co-immunoprecipitation. Wildtype BAF, wildtype emerin (WT, residues 1-222) and mutant emerin proteins (numbered as in Fig. 1), were synthesized and 35S-labeled in vitro using coupled transcription/translation reactions, and then immunoprecipitated using immune (lanes Em, WT, 11-214) or preimmune (pre) antiserum against emerin, or anti-BAF antisera (BAF). In vitro translation of emerin yielded a 27 kDa long form (L), and often also yielded a prominent 23 kDa short form (S) (Östlund et al., 1999), assumed to arise by translation initiation at an internal site, as well as several smaller bands.
