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Fig. 1. Intracellular localization of emerin in HeLa cells. (a-c) Emerin stained with the antibody in fixed cells:(a) interphase; (b) metaphase; and (c) telophase stages of mitosis. (d-e) GFP-emerin in living cells: (d) interphase; (e) metaphase through telophase; time-lapse images in (e) were obtained at one-minute intervals in the same cell. Chromosomes were stained with DAPI in fixed cells, and with Hoechst 33342 in living cells. Bar, 10 µm.
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