Fig. 6. Emerin does not accumulate at nuclear envelopes reassembled in the presence of mutant BAF-G25E. HeLa cells were stained with antibodies against endogenous emerin. (a) A telophase cell expressing GFP-BAF-G25E. (b) Cells expressing GFP-BAF immediately after cytokinesis. (c) An interphase cell that went through mitosis in the presence of GFP-BAF-G25E is indicated by the arrow; cells with no expression of GFP-BAF-G25E are indicated by the arrowhead. (d) A late telophase cell with no expression of GFP-BAF-G25E observed in the same culture dish as for b. (e) HeLa cells expressing GFP-BAF (wildtype) shortly after cytokinesis. Chromosomes were stained with DAPI. Bars, 10 µm.
