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Fig. 11. Fibulin-1 reduces phosphorylation of MHC and ERK. (A) Immunoprecipitation analysis was used to assess [32P]-phosphate incorporation into MHC by MDA MB231 cells cultured for 30 minutes on surfaces of FN or FN-fibulin-1. (B) Immunoblotting was used to study the effect of fibulin-1 on the kinetics of ERK1/2 phosphorylation. The blot shown in B was re-probed with antibodies to non-phosphorylated ERK (C). The data shown in A-C represent at least three experiments each.
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