Fig. 2. Effect of fibulin-1 on FN-mediated cell adhesion. (A) Microtitre wells (tissue-culture polystyrene) were coated with varying concentrations of FN, the unoccupied sites were blocked with BSA and the wells were incubated with either fibulin-1 or BSA (each at 100 µg ml–1). MDA MB231 cells (3.5x104 per well) were incubated with the coatings for 60 minutes at 37°C. The wells were rinsed, cells fixed with 10% neutral formalin and stained with 0.25% crystal violet. The stain was released with 1% SDS and quantified by spectrophotometry. (B) Microtitre wells (non-tissue-culture polystyrene) were coated with varying concentrations of FN and the adhesion of MDA MB231 cells evaluated as above.
