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Fig. 8. Fibulin-1 does not bind to cell surface proteins and does not effect cell surface ß1 integrin binding to FN. Radiolabelled MDA MB231 cell surface proteins were bound to magnetic beads conjugated with fibulin-1, FN or BSA. Before incubation with cells, FN-beads were incubated with either fibulin-1 (FN + fibulin-1) or BSA (FN + BSA). (A) Autoradiograph and (B) immunoblot analysis using anti-ß1-integrin cytoplasmic domain antibody.





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