Fig. 5. Cln2 shares nuclear functions with the S-phase cyclins Clb5 and Clb6, and with the kinesin motor protein Kar3, and shares cytoplasmic functions with the cyclin pair Pcl1 and Pcl2, and with GTPase-activating protein Bud2. (a) The same plasmids as in Fig. 4, the pNE10X series and the pM10X series, were introduced into strain N140 (cln1 cln2 clb5 clb6 GAL-CLN1), and processed as described in Fig. 4. The left panel are serial dilutions that were incubated at 30°C for 2 days, and the right panel were incubated at 34°C for 3 days. (b) Plasmids containing CLN2 with C-terminal forced localization cassettes under control of the methionine-repressible MET3 promoter (pM10X series) were introduced into strain N182 (cln1 cln2 kar3 GAL-CLN1). (c) The same plasmids as in (a) were introduced to the strain N80 (cln1 cln2 pcl1 pcl2 GAL-CLN1), and processed as described in Fig. 4. (d) An empty control vector (pRS314), a low-copy plasmid containing CLN2 with no tag (CLN2/p314M::C2) and plasmids containing CLN2 with C-terminal forced localization cassettes under control of the methionine-repressible MET3 promoter (pNE12X series) were transformed into the strain N163 (cln1 cln2 bud2 GAL-CLN1) and processed as previously described.
