Fig. 5. Detection of the PLV reporter protein by western blot analysis. MML40 cells expressing Rer1p-Cub-PLV were transformed with the plasmids carrying the different Nub fusion proteins, as indicated above each lane. Western blot analysis was performed as described in the Materials and Methods. Protein A was detected with HRP-IgG. The arrow indicates a non-specific degradation product. Lane 1 corresponds to extracts from non-transformed MML40 cells. Mns1p indicates cells expressing Mns1p lacking the Nub in pRS314, and vector indicates cells transfected with pRS314, as negative controls.
