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The life cycle of actin patches in mating yeast

Michael G. Smith, Sudhindra R. Swamy and Liza A. Pon

Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA

Movie 1. Abp1p-GFP patch assembly, movements and disassembly in wild-type, pheromone-treated cells.

Abp1p-GFP-labeled actin patches are visualized in pheromone-treated, wild-type cells. The shmoo tip is located at the top of the image. Optical sections were obtained at 500 nm z-intervals through the entire cell with 100 msec exposure times for each optical section. Using these parameters we obtained 60 consecutive z-series in 65 seconds (1.08 seconds per z-series). Each z-series was deconvolved and rendered into a single 2D projection. All 60 deconvolved, z-series projections are displayed at 10 images/second. Note the retrograde movement of actin patches out of the mating projection tip, and disassembly of motile patches during retrograde movement. The velocity of these movements is 363.48_10.6 nm/second. The average life span of actin patches is 10.9_4.2 seconds.

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