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Fig. 4. Cyclin E localizes to the chromosomes of isolated mitotic spindles. Isolated metaphase (A and C) and anaphase (B and D) spindles were observed using DIC microscopy (A and B) and immunofluorescence microscopy (C and D) using antibodies to cyclin E (shown in red) and {alpha}-tubulin (shown in green). The bright red spots within the spindle asters in panel D are probably fragments of chromosomes. Bar, 10 µm.





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