Fig. 2. Cell division defects in LPCs of dally mutants are rescued by reducing cyclin A expression. (A) Lateral views of brain lobes from dallyP2/TM6B (top left), dallyP2/dallyP2 (top right) and dallyP2 cycA3/dallyP2 (bottom left). cycB/+; dallyP2/dallyP2 (bottom right) third instar larvae are shown stained with anti-cyclin B antibody. The two LPC divisions are seen as two stripes of immuno-positive cells (marked 1 and 2) at the anterior edge of the lamina (LA). Note that the second domain of Cyclin B-positive cells, absent in dallyP2/dallyP2, is observed in the dallyP2 cycA3/dallyP2 preparation. We confirmed that cycB/+ animals show reduced levels but normal patterns of Cyclin B protein expression (data not shown). (B) Quantitative analysis of LPC division rescue by cycA. Third instar larval brains were stained with anti-Cyclin B antibody and scored for the percentage of LPCs showing the second stripe of Cyclin B expression. Each closed circle represents a single larval brain serially sectioned by confocal microscopy, and scored without knowledge of the genotype. cycA5 showed similar effects to cycA3 on expression of LPC division phenotype in dally mutants (data not shown).
