Fig. 5. Pattern of Cyclin A expression in wild-type and dally LPCs. A series of wild-type and dally mutant LPCS in M phase are shown, stained with anti-Cyclin A antibody (red) and propidium iodide (gray scale) to visualize the condensation state of chromosomes. Left panels show Cyclin A staining, middle panels show propidium iodide staining of chromosomes, and the right panels are merges of Cyclin A and propodium iodide images. The top six panels show wild-type LPCs. The arrowheads mark cells where Cyclin A has been degraded and propidium iodide staining shows the cells are in prophase, where chromosome condensation has begun, but not yet achieved the alignment indicative of metaphase. The arrow marks a cell in prophase that retains some residual level of Cyclin A immunoreactivity. dally mutant LPCs show a very different pattern of Cyclin A immunoreactivity in M phase. Shown are several LPCs (arrows) with significant levels of Cyclin A and highly condensed chromosomes characteristic of metaphase. In wild-type Cyclin A immunoreactivity is never seen in LPCs with this degree of chromosome condensation.
