Fig. 1. Amino acid sequences of the N- and C-termini of wild-type GLUT4 and GLUT4 mutants. (A) Predicted topology of GLUT4. CHO, carbohydrate; TAG, position of the HA-epitope tag. (B) Predicted cytoplasmic amino acids of the N-terminus (M1-L24) of human GLUT4 (Fukumoto et al., 1989). (C) Predicted cytoplasmic amino acids of the C-terminus (L466-D509). TM I, TM XII, position of the first and twelfth transmembrane domains. The regions with high homology to the GLUT1 C-terminus (R466-F483; 61% identity, 89% similarity), and the locations of the phenylalanine-5 (F5) and the dileucine-489/90 (LL498/90) motifs are indicated. (D) C-terminal amino acid sequences of the C-terminal truncation mutants (
37, 28).
