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Fig. 9. Pulse-chase analysis of I{kappa}B{alpha} degradation in PC cell lines. LNCaP and DU145 cells were metabolically labeled with 35S-Met-Cys and harvested at indicated time points. I{kappa}B{alpha} was immunoprecipitated, resolved on 12.5% denaturating PAAG and transferred to membrane. Dried membrane was subjected to radiography.





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