Fig. 3. Cell cycle delay of Rad51-overexpressing cells. (A) Simultaneous staining of Rad51 protein (green) and replicating DNA (red) in exponentially growing TGR928.1-9 cells. BrdU was incorporated into DNA for two hours and detected with anti-BrdU antibody. Note that Rad51-foci-positive cells are devoid of BrdU label. (B) Reduced frequency of replicating cells in Rad51-overexpressing lines. Cells undergoing DNA replication synthesis during the last two hours of culture were scored as BrdU+ (black bars), whereas non-replicating cells were BrdU– (white bars). At least 400 cells were analyzed for each experiment.
