Fig. 7. Suppression of p21 and increased etoposide (etop.)-induced apoptosis following antisense inhibition of endogenous Rad51. (A) 400 nM Rad51 antisense (AS) ODN or 400 nM scrambled (SC) ODN or no ODN was used in PPL cells. The cells were then either treated with etoposide or grown in drug-free medium. Relative expressions of p21 and Rad51 were quantified by western blotting. The ß-actin signals (not shown) were used as a loading control. The amounts of Rad51 in untreated PPL and of p21 in etoposide-treated PPL cells, respectively, were chosen as references (100%). For p21, measurements from three independent antisense experiments were averaged. (B) The number of etoposide-induced apoptotic PPL cells after treatment with 100 nM, 400 nM Rad51 antisense or scrambled ODNs, as identified by annexin V staining.
