Fig. 3. Effect of various drugs on the amount of internalized 125I-Tyr3-NT measured by kinetics experiments performed in COS-7 cells transfected with the wild-type mNTR2 (A,C) or the Y237A-mNTR2 mutant (B,D). In cells expressing the wild-type mNTR2 (A,C), the amount of internalized 125I-Tyr3-NT was measured in the absence of drug (closed circles) or in the presence of monensin (open circles), brefeldin A (closed inverted triangles), genistein (open inverted triangles) after acid-NaCl wash and expressed as the percent of total cell-associated ligand at each time. The residual amount of sequestrated-NT was determined in the presence of PheAsO (open diamonds) or sucrose (closed diamonds). In cells expressing the Y237A-mNTR2 mutant (B,D), the internalization of 125I-Tyr3-NT was measured in the absence of drug (closed squares) or in the presence of monensin (open squares), brefeldin A (closed triangles), genistein (open triangles). Each point represents the mean±s.e.m. of at least three different experiments determined in duplicate.
