Fig. 7. Time course of receptor recycling. After induction of receptor sequestration for 15 minutes with 100 nM NT, the peptide was removed by acid washes and the amount of cell surface receptor was determined after indicated times of incubation at 37°C as described in Materials and Methods. (A) Recycling of the rNTR1-WT (n=4), mNTR2-WT (n=6) and hNTR2- WT (n=4). (B) Recycling of the hNTR2-WT (n=4), 1/2-i3Nt-rNTR1 chimera (n=4), the C237Y-hNTR2 mutant (n=4) and effect of the recycling inhibitor monensin on the C237Y-hNTR2 (n=2).
