Fig. 1. Ang2 stimulates autophosphorylation of Tie2. (A) Expression of Tie2 on parental IBE cells was examined by immunoprecipitation with or without immunized peptide, followed by immunoblotting. About 135 kDa single protein was observed. (B) Tie2 was immunoprecipitated from cells (15 cm dishes) either stimulated or left unstimulated with 2 µg/ml Ang2 for 15 minutes and immune complex kinase assay was performed using 90% of cell extracts. Tie2 was immunoprecipitated followed by immunoblotting from the remaining 10% of cell extracts to examine whether a similar amount of Tie2 protein was present in the immune complex kinase assay.
