Fig. 9. Ang2-mediated tube-like structure formation was c-Fyn dependent. IBE cells expressing kinase-inactive c-Fyn (K299M), denoted FynKD-8 cells, were examined by in vitro kinase assay to determine the dominant-negative effect on Ang2 (A). Phosphorylation of acid-denatured enolase was measured by the use of BAS5000 Image Analyzer. FynKD-8 cells were examined for their ability to form tube-like structures mediated by Ang2 as described for Fig. 3B. Representative data were obtained from two independent experiments.
