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Fig. 5. Binding of AP1 and AP2 to peptides representing the cytoplasmic tail of Lamp 1, LAP and LAP{Delta}7. The peptides were immobilised on the surface of a biosensor and probed for the binding of AP1 or AP2 purified from pig brain. From the sensorgrams, the rate constants for association (ka), the dissociation (kd) and the equilibrium (KD) were calculated. It is notable that only the Lamp 1 peptide was able to bind to both adaptors, whereas LAP and LAP{Delta}7 peptides were only able to bind to AP2.





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