Fig. 3. The putative LZ region of TRAX is essential for interactions with C1D and Translin. (A) Yeast two-hybrid assay was performed using DBD-C1D and an activation domain (AD) tagged forms of TRAX; wild-type (WT), N-terminal region containing the LZ motif (N-Ter) or mutLZ where the LZ region of TRAX has been mutated within an otherwise intact protein. The interactions were measured by ß-galactosidase activity using ONPG. (B) Constructs as in (A) were used to detect interaction with the Translin protein expressed as a fusion to the bacterial LexA protein (DBD-Translin). Mutations that disrupt the LZ region of TRAX abolish its interaction with Translin and neither the N-terminal region of TRAX carrying an intact LZ nor the C-terminal region alone (C-Ter) are sufficient for interaction with Translin.
