Fig. 6. Subcellular localizations of TRAX, Translin and C1D. (A) Plasmids expressing CFP-Translin, YFP-TRAX and RFP-C1D were transfected into COS 7 cells and subcellular localizations were determined in the living cells. Translin was mainly cytoplasmic; however, 6% of cells exhibited nuclear/cytoplasmic staining (a). TRAX was nuclear (b) and C1D was mainly nuclear, but a cytoplasmic staining was also evident (c). (B) COS 7 cells were double transfected with the indicated plasmids. (a-c) TRAX/C1D double-transfected cells showing that TRAX is solely nuclear (a) whereas C1D is showing a nuclear/cytoplasmic staining pattern (b), and colocalizes with TRAX in the nucleus (c). In TRAX/Translin double-transfected cells (d-f), TRAX and Translin colocalize in the cytoplasm and in the nucleus. The middle cell in d and f expresses TRAX only. In C1D/Translin double-transfected cells (g-i), C1D is mainly nuclear (g) and Translin is cytoplasmic (h). (C) COS 7 cells triple transfected with TRAX-, Translin- and C1D-expressing plasmids. (a-c) A single cell in which a diffuse nuclear/cytoplasmic staining pattern is observed. In d-f, another field is shown where two of the cells were triple transfected and in which the upper cell TRAX and C1D were expressed in the nucleus (d and f) when Translin was mainly cytoplasmic (e); the lower cell shows a diffuse nuclear-cytoplasmic staining pattern as in a-c. (D) Summary of the results demonstrating the percentage of nuclear/cytoplasmic and solely nuclear localizations of Translin, TRAX and C1D.
