Fig. 1. The GFP-tagged uracil permease is correctly delivered to the plasma membrane. Wild-type cells transformed with p197gF or p195gF-GFP (A) or pFl38gF-GFP (B) and grown on lactate as a carbon source were induced by growth for two hours on galactose medium. (A) Uracil permease activity was followed at various times after addition of galactose. (O) and (
) corresponded to cells transformed with p197gF and p195gF-GFP respectively. (B) Cell fluorescence was followed at various times after addition of galactose. Fluorescence staining was only observed in budding cells. Cells were viewed with a FITC filter set.
