Fig. 2. Deactivation of chemokine-induced migration of lymphocytes (A) and monocytes (B) by AT. Experiments were performed in modified Boyden chambers using nitrocellulose micropore filters. Cells were preincubated with varying AT concentrations for 20 minutes followed by washing. Then chemotaxis of lymphocytes (A) toward Rantes [10 ng/mL] or IL-8 [1 nmol/L] and of monocytes (B) toward Rantes [10 ng/mL] and monocyte chemoattractant protein 3 (MCP-3) [10 nmol/L] was monitored. Results are given as the mean±s.e.m. of the migration index, which is the ratio of the distance of migration (µm) towards attractant and the distance (µm) towards the medium. *, p<0.05, Mann-Whitney U-test versus medium preincubation after multiple group comparison by Kruskal-Wallis test; lymphocytes: p=0.0197 toward Rantes; p=0.0163 toward IL-8; monocytes: p=0.0104 toward Rantes; p=0.0038 toward MCP-3; n=5.
