Fig. 3. Effects of heparinase and chondroitinase treatment on AT-induced chemotaxis. Heparinase I or chondroitinase was added to monocytes or lymphocytes (37°C/5%CO2). After an incubation period of 50 minutes, cells were washed twice and chemotaxis experiments were performed. AT [1 U/mL] served as chemoattractant. Data are expressed as chemotaxis index, which is the ratio between directed and undirected migration. Random migration was 45±3.3 µm for monocytes and 56±2.1 µm for lymphocytes (mean±s.e.m.). For statistical analysis the Mann-Whitney U test was used (*, p<0.05) after the Kruskal-Wallis test (p<0.01); n=3.
