Fig. 5. Effect of thrombin on AT-dependent leukocyte migration. Chemotaxis of PBMC was tested in modified Boyden chambers. The chemotaxis index, which is the ratio between directed and undirected migration of cells, is used to express the data. (A) To test effects of thrombin on AT-induced deactivation of PBMC chemotaxis toward Rantes [10 ng/mL], cells were preincubated in the presence of AT with or without thrombin for 20 minutes. Mann-Whitney U (*, p<0.05) versus control after Kruskal- Wallis (p<0.01); n=3. (B) To test whether thrombin induces cell migration, PBMC were allowed to migrate toward thrombin [100 µU/mL to 1 U/mL]. Mann-Whitney U test (*, p<0.05) versus medium after Kruskal-Wallis (p<0.01); n=3. (C) Cells preincubated with various concentrations of AT followed by washing and migration toward thrombin [10 mU/mL]. Kruskal Wallis test, p>0.05; n=3.
