Fig. 2. Expression and localization of myne-1. (A) A human multi-tissue northern blot hybridized to the 3' UTR of myne-1 is shown. Lane 1, heart; lane 2, brain; lane 3, placenta; lane 4, lung; lane 5, liver; lane 6, skeletal muscle; lane 7, kidney; lane 8, pancreas. The major transcript is 4.2 kb. (B) An immunoblot from murine tissues using affinity-purified AM1 antibody is shown. G, gastrocnemius; Q, quadriceps; H, heart; B, brain; K, kidney; S, stomach; Bl, bladder. Loading control panels are shown below. (C) Immunolocalization using AM1 in heart and quadriceps muscle shows that myne-1 is at the nuclear membrane. Double-labeling with DAPI and AM1 is shown. The left-hand panels represent nuclei visualized with DAPI staining. The right-hand panels are stained with AM1. H, heart; S, skeletal muscle. Bars represent 20 µm.
