Fig. 2. Accumulation of annexin 2-GFP and YFP-tagged S100A10 at sites of EPEC attachment. HeLa cells ectopically expressing annexin 2-GFP (left panels; A,C,E,G) or YFP-S100A10 (right panels; B,D,F,H) were infected with EPEC and then processed for analysis of the fluorescent protein signals (A,B) and rhodamine-phalloidin (C,D), respectively. Merged images of the fluorescent signals are shown in E and F, respectively, whereas G and H give phase contrast images identifying the adherent bacteria. Localization of annexin 2-GFP (A) around polymerized actin (C) at sites of adhering EPEC (G) is indistinguishable from that of endogenous annexin 2. Likewise, YFP-S100A10 (B) is targeted to actin-positive structures (D) beneath EPEC microcolonies (H). The enlarged insets show a 2.5-fold magnification of the boxed region. Bar, 10 µm.
