Fig. 4. HEF1 production increases cell spreading. HEF1.M1 cells were maintained for 18 hours in either non-inducing (A,C,E,G,I) or inducing (B,D,F,H,J) conditions and then replated on glass coverslips coated with 6 µg ml^–1 human FN (1.25 µg cm^–2). Cells were fixed at 30 minutes (A,B), 1 hour (C,D), 2 hours (E,F), 3 hours (G,H) and 6 hours (I,J) after plating. Phase contrast CCD images were acquired with a 40x objective. Cells were maintained in inducing or non-inducing conditions for the duration of the experiment. The graphs show the quantitation of increased cell spreading. HEF1.M1 or CM1 cells maintained for 18 hours in either non-inducing (–) or inducing (+) condition were replated for 6 hours on glass coverslips either uncoated in the presence of 10% FBS (gray) or coated with human FN in serum-free medium (black). CCD images were acquired with a 40x objective (8-10 fields per condition) and the area determined using Inovision ISEE^TM. Results shown are the means of three independent experiments±standard error.

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