Fig. 6. HEF1 production augments FN mediated haptotaxis. (A) Increase in the number of cells that traversed the membrane in a Boyden chamber assay in response to soluble FN, as opposed to the absence of stimulus. HEF1.M1 (black) or CM1 (gray) cells were seeded into the top well of Boyden chambers and assessed for their ability to migrate towards FN in either non-inducing or inducing (for 20 hours) conditions. The haptotactic response of populations maintained in either non-inducing (–) or inducing (+) conditions were grouped separately and normalized against the number of cells that traversed the membrane for each condition in the absence of stimulus. Results shown are the mean of multiple independent experiments for each cell line±standard error. (B) Induced lysates were probed with anti-phosphorylated-MAPK and anti-phosphorylated-p38 antibodies (top), and in parallel with anti-MAPK (lanes 1,2) and anti-p38 (lanes 3,4) (bottom) (C) Inhibition of HEF1.M1 cell haptotaxis toward FN in a Boyden chamber assay (as described above) following treatment with drug inhibitors for MAPK kinase (PD98059, 25 µM), p38 (SB202190, 25 µM) and control (DMSO).
