Fig. 5. Polyacrylamide gel electrophoresis of GV proteins from control oocytes (-hs), oocytes heat shocked at 31.5°C for 4 hours (+hs) and oocytes allowed to recover at 18°C for 12 hours after heat shock (hsR). All oocytes were injected with [³⁵S]-methionine to label newly synthesized proteins. (A) Proteins stained with Coomassie Blue to show equal loading of lanes and the absence of obvious changes in overall protein composition. (B) Autoradiograph of the same samples showing marked reduction in new protein synthesis after heat shock (lane 5) compared with control (lane 4), and renewal of protein synthesis during recovery from heat shock (lane 6). Stars indicate regions of the gel where differences are detectable between the control (lane 4) and heat shock recovery (lane 6). Molecular weight markers are in kDa.

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