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Fig. 1. Immunoblot identification of accumulation of a 160 kDa protein with FN-coated beads. Unbound fractions (lane 1), bead-bound protein complexes (lane 2) from either ConA- or FN-coated beads, and rat brain extract (10 µg) were electrophoresed on an SDS-containing 4-12% polyacrylamide gel under reducing conditions and transferred to nitrocellulose. The membrane was probed with a putative PKC{delta} monoclonal antibody. Indicated on the right are the molecular masses (kDa) of marker proteins.





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