Fig. 4. A pool of histone H1 is localized to the myotube ECM and colocalizes with heparan sulfate proteoglycans. Inhibition of proteoglycan sulfation reduces ECM localization of histone H1. (Left and middle panels) Non-permeabilized myotubes were double-stained with anti-mouse perlecan (per) or anti-rat glypican (gly) antibodies together with an anti-human histone H1 (H1). Phase contrast micrographs of respective fields are also shown (pc). Merged images (H1+per and H1+gly) are also shown. As a control, permeabilized myotubes were stained with the anti-human histone H1 (pH1). TRITC-conjugated anti-rabbit IgG and FITC-conjugated anti-mouse IgG were used as secondary antibodies. (Right panel) C₂C₁₂ cultures were treated with sodium chlorate during differentiation and processed as described for the left panel. Bar, 10 µm.

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