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Fig. 3. GFP-IMP1 localization. (A) Time-lapse microscopy of NIH 3T3 cells that
stably express GFP-IMP1. Cells with GFP-IMP1 undergoing localization are
marked by arrows. (B) Velocity of moving GFP-IMP1 granules. Particles were
followed by time-lapse microscopy at 20 second intervals. The velocities of
100 individual particles were calculated by measuring the distance between the
particles in two succesive frames. (C) Fluorescence recovery after
photobleaching. A cellular extension was bleached, and the transport of
GFP-IMP1 into the bleached area was followed by time-lapse microscopy at 20
second intervals in the absence and presence of sodium azide and
2-deoxyglucose. A similar experiment with GFP-ß-galactosidase is included
as a control.
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