Fig. 8. RNA binding and MAP association are mediated by the KH modules. Autoradiograph from an electrophoretic mobility-shift analysis of ³²P-labelled H19 segment H (H19 seg. H) with recombinant IMP-1 (lane 2: 0.3 nM; lane 3: 3 nM; lane 9: 0.6 nM), RRM1-2 (lane 4: 10 nM; lane 5: 100 nM), KH1-4 (lane 6: 0.6 nM; lane 7: 1.2 nM; lane 8: 3 nM), KH1-2 (lane 10: 20 nM; lane 11: 100 nM) and KH3-4 (lane 12: 20 nM; lane 13: 100 nM). The positions of unbound RNA and RNA-protein complexes a and b are shown at the left. (B) Real-time surface plasmon resonance binding of phosphocellulose-purified MAPs to recombinant RRM1-2 and KH1-4 immobilized on a CM5 sensorchip surface (left panel), and binding of phosphocellulose-purified MAPs and MAP-enriched tubulin to immobilized Drosophila IMP (right panel).

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