Fig. 1. SV40 T antigen transgenic mice and stromal cell generation. (A) The promoters for the constitutively and ubiquitously expressed ß-actin and PGK genes were cloned 5' to the temperature-sensitive SV40 large T antigen (tsA58) gene. Transgenic mouse lines Tag5, containing one copy of the ß-actin tsA58 gene, and Tag11, containing one copy of the PGK tsA58 gene, were used to generate stromal cell lines and clones from embryonic tissues. (B) RT-PCR analysis of RNA from adult Tag5 and Tag11 mice show expression of the tsA58 transgene in all tissues tested. L, lymph nodes; K, kidney; S, spleen; BM, bone marrow, B, brain; M, muscle. (C) The embryonal subregions used for the isolation of stromal clones. E10 and E11 mouse embryos transgenic for the tsA58 gene or a lacZ marker gene were used to generate stromal cell lines and clones from dorsal aorta and mesenchyme (AM), urogenital ridges (UG, mesonephros and genital ridges), the gastrointestinal region (GI) and the liver (EL). Under our culture conditions, no cell lines could be established from the yolk sac. Numbers in brackets indicate the number of clones isolated from the various subregions.

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