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Fig. 5. Localization of actin and vinculin. Cells were centrifuged (35 g)
onto fibronectin-coated slides, and incubated as follows: (A,D) 5 minutes,
4°C; (B,E) 5 minutes, 37°C; (C,F) 2 hours, 37°C. Following
incubation, cells were stained with phalloidin to detect F-actin (A-C) or with
anti-vinculin antibody for immunocytochemical localization of vinculin
(D-F).
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