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Fig. 8. Overexpression of Crb in the amnioserosa disrupts the leading edge cytoskeleton. Lateral view of leading edge of stage 14 GAL4332.3/UAS-crbwt embryo double-stained with anti-nonmuscle myosin heavy chain antibodies (A) and anti-phosphotyrosine antibodies (B). Phosphotyrosine and myosin are depleted in patches along the leading edge (arrowheads), and there is little elongation of epidermal cells. Compare the leading edge myosin staining in A with the wild-type leading edge myosin staining in Fig. 3C.





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