Fig. 4. Cell-type-specific immunostaining for neurons (A,B), astrocytes (C,D) and oligodendrocytes (E,F) in CB-derived cells growing in differentiation-promoting media. (A,B) Cells expressing class III ß-tubulin recognised by the TUJ1 antibody and displaying neuron-like morphology with long axonal projections. The filamentous feature of cytoplasmic structures that are immunoreactive with the TUJ1 antibody and corresponding to neuronal microtubules are clearly visible under higher magnification (B). (C,D) Cells immunostained with the anti-GFAP antibody. Some of the cells are round and relatively small, whereas others contain long projections with immunoreactive filamentous structures that are visible in the cytoplasm (D). (E,F) GalC-immunoreactive cells expressing galactosylceramides and displaying morphology characteristic of oligodendrocytes, with flat cell body and short or long branched projections. Smaller, round immunoreactive cells are also occasionally present (see the right upper corner). Bars: A,C,E, 50 µm; B,D,F, 20 µm.

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