Fig. 5. Neural differentiation of CB-derived cells after their plating on the monolayer of rat primary cortical culture. The CB-derived cells (coloured green by the `cell tracer' in A,E,I and by Texas Red after phenotype-specific immunoreactions in B,F,J) can be detected in the vicinity of cells originating from rat cortex (only red). Both stains were detected simultaneously (C,G,K) and together with nuclei of all cells forming rat primary cortical monolayer revealed by the use of Hoechst 33258 staining (D,H,L). Colocalisation of red and green labelling in C,G,K and D,H,L appears yellow after overlaying these two images. (B,C,D) Neuron-specific immunostaining for type III ß-tubulin with TUJ1 antibody. (F,G,H) Astrocyte-specific immunostaining with anti-GFAP antibody. (J,K,L) Oligodendrocyte-specific immunoreaction with galactosylceramide recognised by the GalC antibody. Arrows indicate immunopositive CB-derived cells. Scale bars for A to H shown in A and E correspond to 50 µm. The scale bar for I to L shown in I corresponds to 20 µm.

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