Fig. 7. Examples of phenotypic diversity among a progeny of CB-derived neural precursors induced to differentiate in various culture conditions. (A) Overlaying images of expression of two neuron-specific proteins: class III ß-tubulin (immunostained by a Texas-Red-conjugated secondary antibody) and MAP2 (immunostained green by afluorescein-conjugated secondary antibody). The TUJ1-positive structures, which are also immunoreactive with the anti-MAP2 antibody, appeared yellow when detected simultaneously (arrows). Some of these cells display characteristic neuron-like morphology (arrowhead). Cell nuclei are blue contrastained by Hoechst 33258. (B) Distinct distribution of neuron- and astrocyte-specific proteins is indicated by MAP2 (green) and GFAP (red) immunostaining, respectively. Cell nuclei are blue-stained by Hoechst 33258. Very fine, green, anti-MAP2 immunoreactive cytoskeletal filamentous structures can be seen in the cytoplasm of neuron-like cells. (C) CB-derived cells prelabelled green by `cell tracer' reveal the typical morphology of matured oligodendrocytes with long, branched projections expressing GalC-immunoreactive galactosylceramides. In the figure, fragments of green-traced cells positive for GalC (recognised by Texas-Red-conjugated secondary antibody) appear yellow owing to the overlaying of these two colours when detected simultaneously.

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