Fig. 4. PKC isozyme localization in myoblasts plated on FN. Myoblasts were plated on FN for various times, methanol/acetone fixed and stained for individual PKC isozymes. This figure shows characteristic patterns of localization of , and PKC as the cells attach and spread. These results were observed in more than 90% of the cells. PKC is localized at focal adhesion sites (arrow) after 15 minutes on FN. After 15 minutes on FN, PKC revealed a Golgi-like staining (arrow), whereas after 1 hour, PKC showed a punctate staining pattern at the cell periphery (arrow). After 15 minutes, PKC was detected in the nucleus and in perinuclear regions (arrow), and became localized diffusely in the cytosol as well as in the nucleus after 1 hour on FN.

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