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Fig. 7. Actin reorganization in
5-expressing cells plated on FN. Actin
localization and stress-fiber formation were observed in
5-expressing
cells plated on FN. Paraformaldehyde-fixed cells were stained for actin and
stress fibers (F-actin) as indicated in the Materials and Methods. At early
time points (15 minutes), actin was found at focal adhesion sites (arrow).
With time, focal contacts were distributed uniformly across the cell surface,
and fine stress fibers were found around the nucleus and at the periphery of
the cell.