Fig. 1. H-ferritin immunostaining in a human astrocytoma tumors. A total of six different tumors were analyzed. The sections in A-D represent contiguous sections from the same tumor. Note the blood vessel containing red blood cells in the upper left-hand corner for orientation. (A) H-ferritin was detected with the HS-59 monoclonal mouse anti-human rH-ferritin antibody (red immunoreaction product). The arrows indicate examples of positive cell nuclei. (B) A contiguous section was reacted for H-ferritin (red) and a blue counterstain was applied to show the presence of cell nuclei. An example of a ferritin positive nucleus is indicated by the arrow. A non-ferritin positive nucleus is indicated by the arrowhead and appears blue. (C) This section was stained for Mouse IgG as a nonspecific control for the secondary antibody. No staining is visible, although nuclei can be seen in the field (arrow). (D) This section was counterstained to reveal the cell nuclei (blue, arrow) after incubation with mouse IgG. (E) This section represents GFAP staining in the human astrocytoma. Intermediate (GFAP-positive) filaments are seen coursing throughout the cytoplasm (red). The section was counterstained to visualize the nuclei that appear blue (arrow). The staining with GFAP represents a control for nuclear ferritin staining with a different human monoclonal antibody that would stain cellular features of the astrocytoma. (F) An immunoreaction for neurofilament was performed on the astrocytoma as an irrelevant control monoclonal antibody. There is no red reaction product for neurofilaments. The section was counterstained blue to reveal nuclei (arrow). Bar, 10.8 µm.

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