Fig. 6. ¹²⁵I-rH-ferritin crosslinks to DNA in whole cells. ¹²⁵I-rH ferritin was incubated with SW1088 astrocytoma cells grown in standard, untreated medium as a control (CN) or in the presence of 100 µM deferoxamine (DFO). (A) One group of cells from both CN and DFO conditions were placed in 1% formaldehyde to crosslink DNA-protein complexes (lanes 3 and 4). The other group was not subjected to crosslinking (lanes 1 and 2). (B) The membranes containing the crosslinked complexes were incubated with a rH-ferritin-specific antibody to demonstrate that the crosslinked protein was ferritin. Lane 1, CN; lane 2, DFO; lane 3, CN crosslinked; lane 4, DFO crosslinked. (C) The numerical values obtained from the densitometric analysis of each lane in A are shown. The data shown in the figure are representative of three different experiments. ¹²⁵I-rH ferritin-DNA complexes were identified in CN and DFO crosslinked conditions with the greatest level found in the DFO group.

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