Fig. 6. Cellular endocytosis of truncated versus latent heparanase. Human foreskin fibroblasts were incubated with 10 µg/ml 65 kDa heparanase (right panels) or with 10 µg/ml processed 50 kDa heparanase (left panels) for 30, 60 and 120 minutes. After fixation and permeabilization, immunofluorescent staining was performed with anti-heparanase mAb 130. Heparanase-containing granules were detected and quantified as described in the Materials and Methods. Uptake of the processed enzyme was significantly faster. The intensity of the immunoflourescent staining, size and the number of processed heparanase-containing granules reached saturation after 30 minutes.

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